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The Applied Biocatalysis group includes projects for the recombinant production of biocatalysts, metabolic engineering and the use of enzymes or whole cells for the synthesis of chemical molecules.
Our scientific interest is the production of "difficult to express" proteins in Escherichia coli and other prokaryotic hosts. These include multisubunit proteins, superlarge enzyme complexes or peptides. Optimisation strategies are directed to proper folding of the proteins of interest with a special focus on posttranslational modifications, such as disulfide bond formation.
For the selection of the biocatalysts and successful process development we apply the methods available from our Automated Bioprocess Development Facility, which guarantee a fast and consistent developmental process.
The current proteins of interest include (i) enyzmes in the the nucleoside and nucleotide metabolism for the synthesis of non-natural bioactive compounds and (ii) the synthesis of ribosomal and nonribosomal bioactive peptides.
One of the innovative biocatalysis applications has been the development of the enzyme based substrate delivery systems (EnBase®). This technology offers a novel approach to control the supply of substrates to obtain high cell density cultures. Thus the technology imitates a fed-batch culture without the use of mechanical pumps and thus it is scaleable from μl to large scale. The technology is jointly developed with BioSilta Ltd.
Metabolic engineering of cells for the synthesis of bioactive peptides